To collect a midstream "free catch" urine sample from an animal, when should you collect the sample?

Collecting a midstream "free catch" urine sample involves obtaining urine after the animal has begun to urinate but before it finishes. The purpose of this method is to ensure that the sample collected is less likely to be contaminated by bacteria or debris that may be present around the urethra and in the initial portion of the urine stream. By waiting until the animal has urinated a little, you allow the urine to flush out any contaminants, which helps ensure that the sample reflects the state of the bladder rather than external contaminants.

The other options do not align with the goal of obtaining a clean and representative sample. Collecting urine before the animal starts urinating would not yield any sample. Waiting until the animal is calm or timing the collection immediately after feeding does not specifically address the need to collect the midstream portion.